Estimation of Spectral Transmittance in Optical Microscopic Image and its Applications
نویسندگان
چکیده
Spectral transmittance in optical microscopic image was estimated from multi-band image taken through five broad band filters based on Wiener estimation method. Matrix for Wiener estimation was calculated using the 16 spectral transmittances of color transparencies measured in advance by spectral photometer. The optical microscope adjusted for monochrome digital camera (Kodak DCS420m, 1536x1024 pixels) with five band filters was used for image acquisition. The fungi image taken by those imaging systems were analyzed by using the estimated spectral transmittances.
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